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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in many tumors, which ends up in chromosome instability, and so may possibly add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential job in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two added splice variants had been documented but detailed nucleotide sequence information and facts is just not however available.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide generally has the epitope regarded from the antibody.

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This guideline outlines additional probable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...

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This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to make sure the absolute best effects are...

Antibodies sure to the blocking peptide no longer bind into the epitope within the focus on protein. By comparing the staining from the blocked antibody vs . the antibody on your own, you can see which staining is certain.

Target Info Cyclin E belongs on the extremely conserved cyclin spouse and children, whose associates are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation designs which add towards the temporal coordination of each mitotic function. Cyclin E kinds a fancy with and capabilities as a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary which is degraded as cells development by S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each check here antibodies...

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